25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. • Suitable for use in 96- and 384-well plate formats. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The PG reagent from the Quant-iT TM PicoGreen TM dsDNA Assay Kit (Invitrogen) was prepared immediately before use by making a 1:200 dilution of the PG in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7. Quant It Picogreen Dsdna Assay Kit Reagents, supplied by Thermo Fisher, used in various techniques. Thermo Fisher Scientific公司的Quant-iT PicoGreen dsDNA 检测试剂盒对DNA更特异，比传统光吸收方法的灵敏度高约 1000倍。 如产品手册所述，此实验在微孔板格式中采用单 一染料浓度的动态范围从250 pg/mL到1000 ng/mL。在这 里，我们证明了使用Molecular Devices公司SpectraMax®Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. 50 pg to 2 µg. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The assay is performed at room temperature, and the signal is stable for 3 hours. Quant-iT PicoGreen dsDNA Assay Kit. Application observe demonstrating so with Molecular Devices SpectraMax® microplate book and the Quant-iT PicoGreen assay, users can reliably size concentrations as low as 50 pg/mL of double-stranded DNA. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. The Quant-iT™ dsDNA High-Sensitivity Kit is designed for assaying samples containing 0. ” J Pharm Biomed Anal. Our Quant-iT™ PicoGreen® dsDNA reagent enables researchers to quantitate as little as 25 pg/mL of dsDNA (50 pg dsDNA in a 2 mL assay volume) with a standard spectro fluoro- meter and. 5 ng/ml of dsDNA were detected with minimal effect in the presence of ssDNA, RNA, and free nucleotides, which is comparable with InvitrogenTM Quant-iT™ PicoGreen® dsDNA Reagent. All LNP samples were diluted 300-fold in TE buffer and placed on a Corning ® 96 Well Solid Polystyrene Microplate (Sigma-Aldrich, St. g. 5 mm in the Eppendorf BioSpectrometer fluorescence, PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Picogreen assays are compatible with most microplate readers and fluorometers. P7589. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. Therefore, fluorescence spectroscopy using PicoGreen is more useful than UV spectroscopy for evaluating the template activity of DNA for PCR. Got it. 99 to $1999. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. Begin typing to search, use arrow keys to navigate, Enter to select. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 8. o cDNA quantification using Quant-iT PicoGreen dsDNA Assay Kit (P7589, Life Technologies) o PCR Cycles used as indicated in NUM_PCR_CYCLES_cDNA and NUM_PCR_CYCLES_Nextera x C1_ADD_Reagents: o Randomer added to Lysis Reaction Mix, (3. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo. PicoGreen Assay: CHO DNA Concentrate (10 µg/mL), 120 µL D553X Quant-iT PicoGreen dsDNA reagent D003 Solution, 1 x 1. The Quant-iT PicoGreen dsDNA. dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. QuantiFluor® ONE dye sensitivity range is 0. Picogreen assays are compatible with most microplate readers and fluorometers. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. Picogreen assays are compatible with most microplate readers and fluorometers. The Rapidly detect as little as 0. Bioz Stars score: 86/100, based on 1 PubMed citations. 2-200 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iTTM DNA Assay Kit, Broad Range 2-1000 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iT™ DNA Assay Kit, High Sensitivity 0. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 产品概述. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. 9, 10 The Pico-Green assay is sensitive to 25 pg dsDNA/mL, which is 400-fold more sensitive than Hoechst 33258 dye. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 2 DNA selectivity and sensitivity of the Quant-iT dsDNA high-sensitivity assay. Fluorometric dsDNA Assay. 5) prepared from 20x. It must be completely liquified before use by allowing it to come to room temperature. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. Because the PicoGreen dye is an extremely sensitive detection reagent for dsDNA, it is imperative that the TE buffer is free of contaminating nucleic acids. • Suitable for use in 96- and 384-well plate formats. The Quant-iTTM PicoGreenTM dsDNA Reagent enables researchers to quantitate as little as 250 pg/mL of dsDNA (50 pg dsDNA in a 200 μL assay volume) with a fluorescence. Per Well Volume 150 uL/well. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Prepare the 200 fold dilution of pico dye to be kept in the dark. The fluorescence intensity of PicoGreen was measured to determine the. Rapidly detect as little as 0. • Specific for dsDNA in the presence of RNA. Using 20x TE buffer make 1x solution. The linear detection range of the PicoGreen® assay in the 20/20n extends over three orders of magnitude in DNA concentration - from 450 pg/mLFor DNA quantification with PicoGreen (PG), Quant-iT™ PicoGreen® dsDNA Reagents and Kits (Invitrogen – Molecular Probes, Eugene, OR, USA) were used. DNA standards (λ DNA) and DNA samples were stained using a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Eugene, OR, USA). the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. For example, if the amount of the linear dsDNA library available is 20 ng, the linear dsDNA library input should be 10 ng, and the linear dsDNA library concentration should be at least 0. Accuracy of the Qubit dsDNA HS assay compared to the Quant-iT PicoGreen dsDNA assay plotted on two different types of curves. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. Quant-iT PicoGreen dsDNA Assay Kit. Fluorescence was measured at. Thermo Fisher quant it picogreen dsdnaassay kit Quant It Picogreen Dsdnaassay Kit, supplied by Thermo Fisher, used in various techniques. 适用于（设备）. You will need 100 ul diluted PicoGreen per well containing 100 ul sample. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. This protocol is a simplified and condensed version of. Short-read sequencing was performed on an Illumina NextSeq 2000 platform (2x151bp. • Suitable for use in 96- and 384-well plate formats. Picogreen assays are compatible with most microplate readers and fluorometers. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. quant it picogreen dsdna assay kit reagents. Determine the DNA concentration on the basis of absorbance at 260 nm (A 260) in a cuvette with a 1 cm pathlength; an A 260 of 0. Showing 4 of 4. , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Quantitation Range. Product Type. Rapidly detect as little as 0. 2–100 ng, the fluorescence signal is linear with DNA. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). '. 86. Absorbance-based measurements employ the DNA as it is and volumes of 100 µl, 50 µl, and 2 µl were placed into a 96 well, 384 well, or LVis plate, respectively. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. 激发/发射. Natural cures with herbs are. (An additional 1 ml TE with 5 ul PicoGreen may need to be prepared for the standards. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for. Contents and Storage Information. Equal amounts (100 µL) of working PicoGreen quantitation reagent were mixed and incubated for 10 minutes at room temperature, protected from light. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. 995 mL TE for the . 3. 10 ng/mkl – 10000 ng/ml. • Suitable for use in 96- and 384-well plate formats. This was possible since, due to the low height of the light ray of only 8. Product Type. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was added to 199 μl of a Qubit working solution. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0. This is Standard 1 which will have a final concentration of 1 ug/mL. PicoGreen dsDNA quantitation reagents are. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. with the Qubit dsDNA BR Assay Kit and 1 µL of each sample was measured in quadruplicate with the Quant-iT™ PicoGreen dsDNA Assay Kit. 1 mL kit. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. This protocol is a simplified and condensed. Figure 2. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. All NGS libraries generated with both the Fragmentase and the FTP method contained similar amounts of ds DNA (800 ± 50 ng) and had similar mean insert sizes of the libraries in a range from 400 to 500 bp. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. 9. 99. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. このアッセイは、従来のUV吸光度法に比べて感度が何倍も高いため、様々な濃度範囲に対して最適化することが可能です。. Spectrofluorometry was performed based on guidelines from ThermoFisher Scientific Inc. The libraries were sequenced using the Illumina HiSeq 2500. Each Helixyte™ assay kit is specially formulated with a target-selective DNA. This assay is designed for use with fluorescence microplate readers. Highly selective for dsDNA over ssDNA or RNA. Description. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Picogreen assays are compatible with most microplate readers and fluorometers. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Le réactif et les kits de quantification d’ADNdb PicoGreen sont parfaits pour les dosages PCR, les échantillons de biopuces, les dosages de lésions de l’ADN, les dosages d’activité enzymatique, la quantification d’ADN génomique, la mesure de l’ADNdb dans des mélanges complexes et la quantification d’ADN viral. P11495. • Specific for dsDNA in the presence of RNA. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. P7589. 500/525. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitlimit consume only 2 picograms of dsDNA per measure-ment. 3 Q33120 39. Rapidly detect as little as 0. Picogreen assay kit (Invitrogen, P7589) a. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT™ PicoGreen™ dsDNA Assay KitInvitrogen - Thermo FisherCatalog #P11496 Quant-iT™PicoGreen® dsDNA reagent is dissolved in dimethylsulfoxide (DMSO), which freezes below 19 °C. 双链DNA（dsDNA）定量检测试剂盒 【产品升级声明】： 我司（懋康生物）从22. Control samples were not lysed and consisted of free pDNA. Qubit® dsDNA HS Assay Kit. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. 1 Warm Quant-iT PicoGreen reagent to room temp in the dark. If your are using plastic cuvettes or assay plates, reduce this problem by including 0. 10 x 100 µL. The final pooled sample was quantified using a Quant-IT PicoGreen dsDNA assay (Thermo Fisher Scientific) and the resulting fragment size distribution was assessed using a Bioanalyzer (Agilent. Table 2 summarizes results of the. Rapidly detect as little as 0. Helixyte™ Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. Helixyte™ Green exhibits large fluorescence enhancement upon binding to dsDNA. PicoGreen dsDNA quantitation reagents are. Sequencing libraries were built using Illumina's TruSeq Nano DNA LT Library Prep Kit, and the NovaSeq 6000 SP Reagent Kit (500 cycles) was used. 50 pg to 2 µg. Picogreen assays are compatible with most microplate readers and fluorometers. Qubit assays are available for double- and single-stranded DNA (dsDNA and ssDNA), RNA, microRNA, and protein. Rapidly detect as little as 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 3 Q32854 26. It requires the most user preparation and calculation, but can be adapted for cuvettes or plates. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. 2–100 ng of DNA. All cfDNA were quantified by QUBIT dsDNA HS Assay kit (Thermo Fisher Scientific®, Aalst, Belgium), according to the manufacturer’s data. A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. Rapidly detect as little as 0. 2023-11. PicoGreen定 量检测方法简单、方便，被多家生物制品厂所选择，成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光，无DNA不发荧光；所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出，PicoGreen定量DNA的方法检出限约0. Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of ssDNA and RNA with minimal effect on the quantitation results. 7. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 21 DiB) [22]. Quant-iT OliGreen ssDNA Assay Kits contain an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. The lambda DNA standard, provided at 100 µg/mL in the Quant-iT ™ PicoGreen dsDNA Assay Kit, is diluted 50‑fold in TE to make the 2 µg/mL working solution. in the assay in order to reduce waste and still achieve comparable results. Nucleic acid quantitation using Quant-iT Reagents. P11496). ZERO BIAS . 99 to $1999. The stock DNA and the corresponding 1:5 diluted DNA derived from FFPE tissue was quantified by using Quant-iT™ PicoGreen dsDNA Assay kit (Thermo Fisher Scientific) as follows: 2 μl of sample DNA were diluted 1:5 vol/vol in 1× TE, containing 1:100 diluted PicoGreen reagent in a final reaction volume of 40 μl per reaction. After quantitation, the purified amplicons from each reaction mixture were mixed at equimolar ratios based on concentration and subjected to. (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation. Quantitation Range. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Technical note | Quant-iT and Qubit 1X dsDNA assaysthe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Product Type. Selection guide for the Qubit and Quant-iT Assay Kits—Table 8. 86100 stars. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). ) Add PicoGreen to DNA samples Add 100 1x PicoGreen to each well of the DNA/standard plate without contaminating tips. The tubes were. Unit Size. Figure 8. Q33120). Quant-iT dsDNA BR buffer (Component B) 250 ml c. Library quality and average fragment size was assessed using a Bioanalyzer (Agilent, G2939A) High Sensitivity DNA chip (Agilent, 5067-4626). 20 and 2. 99 to $1999. g. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 99 to $1999. • Suitable for use in 96- and 384-well plate formats. PicoGreen®試薬アッセイは、蛍光を基にした迅速かつ簡単な手法により、dsDNAを正確に定量することができます。. PicoGreen®試薬アッセイ. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and the Quant-iT Broad-Range dsDNA Assay Kit provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. PicoGreen dsDNA Assay Kit. is 1 pg/μL – 5 ng/μL so the sensitivity of this kit can be rated as Ultra High (UHS). 6 Add equivalent volume (100 μl) of diluted PicoGreen to every well (keeping plate in the dark as much as possible). Rapidly detect as little as 0. The method for this assay follows the instructions in the product information sheet for Quant-iT PicoGreen dsDNA Reagent and Kits from Molecular Probes, except that the assay volume is proportionately reduced from 2. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. 4 mL of prewarmed to 37°C, TE Buffer Cat# D001 or other qualified TE buffer. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Picogreen assays are compatible with most microplate readers and fluorometers. • Specific for dsDNA in the presence of RNA. Figure 2. 2023. This reagent is also included with the Quant-iT RiboGreen RNA Assay Kit and comes complete with buffer and RNA. Quant-iT PicoGreen dsDNA Assay Kit. DNA samples with a concentration determined by 260 nm absorbance higher than 100 ng/mL were diluted. 8 List of Tables Molecular Probes amine-reactive dyes—Table 1. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 99. 2. Library quality and average fragment size was assessed using a Bioanalyzer (Agilent, G2939A) High Sensitivity DNA chip (Agilent, 5067-4626). Alternatively, purified RCA products can be quantitated by measuring the absorbance at 260 nm or by NanoDrop®. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Materials: 1. Reagents – The Quant-IT™ PicoGreen® kit was purchased from Thermo Fisher Scientific. Description. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Reagent Search & Data Services Menu Reagent Search. DNA standard curve. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. Buy from Supplier. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 2 – 100 ng. Qubit™ dsDNA Quantification Assay Kits. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Rapidly detect as little as 0. 2 to 100 ng) Assay Kit were used with a Qubit 3. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. Quant-iT PicoGreen dsDNA Reagent. The Quant-iT™ dsDNA BR assay can be adapted for use in cuvettes or 384-well microplates. Qubit® dsDNA BR Assay Kit. PicoGreen dsDNA quantitation reagents are. Libraries were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies) and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). low-range. Generation of a standard curve using dsDNA-Standards Preparation of the measurement solution was based on the user manual of the Quant-iT, PicoGreen dsDNA kit. Quantitation Range. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. 线，利用这一曲线并根据经验检测样本的浓度。Qubit dsDNA HS Assay可用于测量DNA浓度在10pg/µL 至100ng/µL 之间 的样本。超过此浓度范围的样本必须经过稀释然后进行重新 测试，以确保它们的结果落在参考标准的线性范围内。Qubit dsDNA HS Assay具有高度的准确. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. quant it picogreen dsdna assay kit , 2023-10. 특징: Quanti-iT Picogreen을 이용하면 낮은 농도의 DNA 측정에 유리. Determination of DNA Yield. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume). Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Sample fluorescence was. Sample cDNA concentration was measured using the Quant-iT PicoGreen dsDNA assay kit and black Nunc F96 MicroWell polystyrene plates (ThermoFisher Scientific) according to the manufacturer’s. 1 mL kit. Unit Size. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. PicoGreen dsDNA quantitation reagent is a highly sensitive fluorescent nucleic acid stain which is used to quantitate double-stranded DNA. 2000 ng/mL of lamda dsDNA was serially diluted in 1XTE before the addition of the PicoGreen working solution. Rapidly detect as little as 0. Calibrator MPO. 1X dsDNA assay (%) 100 17. The Quant-iT RNA Reagent and Assay Kit makes RNA quantification easy and accurate. DNA was normalized to 2 concentrations, 100+/-20µg/mL and 20+/-5µg/mL. No. Rapidly detect as little as 0. Figure 8. Optimized for fluorescence microplate readers. Rapidly detect as little as 0. 19. Prepare only the amount of 1:25 diluted PicoGreen required for that run. The ITS protocol detailed here is designed to amplify fungal microbial eukaryotic lineages using paired-end community sequencing on the Illumina platform with primers ITS1f-ITS2 (EMP. 1 mL kit. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. 3ng/mL，DNAThe fragment size of the amplicon was assessed using agarose gel electrophoresis and quantified by Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). Material Amount Concentration Storage Stability Quant-iT™ PicoGreen® dsDNA reagent (Component A) 1 mL in 1 vial (P7589) or in10 vials of 100 μL each (P11496) Solution in DMSO 2–6ºC Desiccate Protect from light • • • When stored as directed, product stable. Coleen - 06 Oct. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). 5 ng/ml ~ 1,000 ng/ml 까지 정비례. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. P11495. Genomic DNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. assay. PicoGreen dsDNA quantitation reagents are. 7 Q33232 4 Green features Less waste and use of fewer resources With the Qubit and Quant-iT 1X dsDNA HS and 1X dsDNA BR Assay Kits, instead of supplying the assay reagent and dilution buffer in separate bottles as was done with the. Samples should be free of RNA contamination and the use of the Qiagen Reaction Cleanup Kit (Qiagen) is recommended. Picogreen assays are compatible with most microplate readers and fluorometers. Libraries were sequenced on the Illumina NovaSeq. This protocol is a simplified and condensed. io |. PicoGreen dsDNA quantitation reagents are. Easy to use UV absorbance alternative assay that can be. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. (Waltham, MA, United States) using the Quant-iT TM PicoGreen TM dsDNA Assay Kit. Average size fragment was determined using a LabChip GX (PerkinElmer) instrument. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. Picogreen assays are compatible with most microplate readers and fluorometers. 2. 使用 PicoGreen dsDNA 定量试剂盒和试剂时，即使在存在 ssDNA、RNA 和游离核苷酸的情况下，也可快速检测低至 0. A 96 plate well was filled with 100 µl of dye and 100 µl of DNA, a 384 well with 10 µl of each solution. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267dsDNA in the presence of equimolar concentrations of ssDNA and RNA with minimal effect on the quantitative results. For duplicate-well droplet. Bioz Stars score: 99/100, based on 1 PubMed citations. Abstract. 0 ml) of the aqueous working solution of Quant-iT™ PicoGreen dsDNA Reagent was added to each cell sample, which was incubated for 10 min at 37 °C in the dark. 25 pg/uL 的 dsDNA。 该定量试剂盒在三个数量级内呈线性，几乎无序列依赖性，可让您准确测定多种来源的 DNA，包括基因组 DNA、病毒 DNA、小提 DNA 或 PCR 扩. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Working PicoGreen reagent was prepared by diluting the concentrated DMSO-PicoGreen stock solution, provided in the PicoGreen kit, 1:200 with TE according to the kit instructions. The original sequencing data. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM 製品番号（カタログ番号）： P11495. If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. Cloning, expression and proteins purification. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Mix by gently vortexing for 5 seconds. 2–100 ng, the fluorescence signal is linear with DNA. 99. VIEWS. Rapidly detect as little as 0. dsDNA and PicoGreen analyzed using the Modulus and the Blue Fluorescence Optical Kit. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitQuant-iT™ PicoGreen® dsDNA Reagent and Kits | 4 Experimental Protoco l DNA Standard Curve 1. The accuracy index is defined in Table 2. Picogreen Assay for DNA quantification: Which one is better to go for, a fresh. Rapidly detect as little as 0. 0, 20X c. RESULTS The OT-2 Constructs Highly Uniform LibrariesThermo Fisher double stranded dna quant it picogreen dsdna assay kit Double Stranded Dna Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Fluorescence signal measured for the DNA sodium salt standard from calf thymus using the Quant-iT™ PicoGreen® assay. The kit is robust with a mix and read format compatible with 96- and 384-well fluorescence-based microplate readers. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). 50 ul PicoGreen Mix by swirling bottle. Sequencing was performed with a HiSeq Single-End Illumina platform (USA) [Citation 31]. Learn how to use the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit to quantitate double-stranded DNA (dsDNA) in solution with a fluorometer or a microplate reader. Picogreen assays are compatible with most microplate readers and fluorometers. The range of linearity for DNA concentration measurements. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Pico488 allows to quantitate as little as low pg/mL of dsDNA. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. 使用 Quant-iT Picogreen dsDNA 定量试剂盒和 dsDNA 试剂在较宽的动态范围内实现精准的 dsDNA 浓度测定。. The Qubit dsDNA BR Assay kit contains a highly selective enhancing reagent specific for quantifying dsDNA using volumes as low as 1 µL in a standard 96-well microplate format. To perform fluorometric quantification of cfDNA quantity Quant-iT PicoGreen dsDNA Assay Kit (Thermofisher), was used, following the manufacturer’s instructions. PicoGreen dsDNA quantitation reagents are. Table 2 summarizes results of the. Rapidly detect as little as 0. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. Picogreen assays are compatible with most microplate readers and fluorometers. Technical Support Customer Service. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA.